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General Agriculture & Tools

6-Benzylaminopurine (6-BAP): Cytokinin Growth Regulator

6-Benzylaminopurine (6-BAP, also known as Benzyladenine) is a synthetic first-generation cytokinin plant growth regulator widely employed in plant tissue culture, commercial floriculture, and fruit farming. Operating at cellular levels, 6-BAP stimulates active cell division (cytokinesis), breaks lateral bud dormancy, promotes shoot initiation, and delays foliar senescence. Growers utilize 6-BAP to enhance branch proliferation, improve fruit set and sizing in apple and grapes, and preserve green freshness in post-harvest leafy crops.

Last reviewed: 10 November 2026

Cytokinin hormonal mechanism and cellular stimulation

6-BAP acts directly on plant cell cycles to regulate meristematic growth and nutrient mobilization.

  • Promotes cytokinesis: Accelerates plant cell division in apical and lateral meristems.
  • Overcomes apical dominance: Releases dormant lateral buds to develop into productive fruiting shoots and dense bushy canopies.
  • Delays foliar senescence: Retards chlorophyll breakdown and protein degradation, keeping leaves green and photosynthetically active.
  • Nutrient sink mobilization: Directs sugars, amino acids, and minerals toward developing flower buds and fruit clusters.

Commercial applications in fruit orchards and horticulture

Targeted foliar applications of 6-BAP at ultra-low concentrations optimize crop canopy architecture and fruit quality.

  • Apple production: Used as a post-bloom chemical fruitlet thinner and to improve fruit typiness, elongation, and size.
  • Grape cultivation: Enhances berry set, rachis elongation, and uniform berry sizing in seedless table grape varieties.
  • Floriculture and ornamental plants: Stimulates basal branching in roses, carnations, and chrysanthemums for higher stem counts.
  • Vegetable crops: Delays yellowing of leafy greens (spinach, coriander) and enhances fruit set in greenhouse cucurbits.

Plant tissue culture and micropropagation techniques

In commercial biotechnology laboratories, 6-BAP is an essential cytokinin added to Murashige and Skoog (MS) media to stimulate rapid multiple shoot regeneration from explants (such as banana shoot tips and sugarcane meristems). Because 6-BAP is biologically potent at parts per million (ppm) levels, applicators must dissolve it in dilute alkali or alcohol before water dilution.

Official source

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National Horticulture Board (Govt of India) ↗

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Common questions

What is 6-Benzylaminopurine (6-BAP) used for in plant agriculture?

6-BAP is a synthetic cytokinin plant growth regulator used to stimulate cell division, promote shoot branching, enhance fruit sizing, and delay leaf yellowing.

Why is 6-BAP widely used in plant tissue culture laboratories?

It stimulates rapid shoot bud differentiation and multiple shoot proliferation from explants on culture media.

How does 6-BAP benefit apple and grape growers?

In apples, it helps with fruitlet thinning and sizing; in grapes, it enhances rachis stretching and uniform berry development.

General guidance, not personal advice. For your farm, ask your local Krishi Vigyan Kendra or agriculture officer.

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